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目的观察乌司他丁对庆大霉素损伤的大鼠肾小管上皮细胞表达内皮素-1(ET-1)和一氧化氮(NO)的影响。方法传代培养大鼠肾小管上皮细胞(NRK-52E),分为对照组、损伤组(2 mg/ml庆大霉素)、治疗组A(2mg/ml庆大霉素+160 U/ml乌司他丁)、B(2 mg/ml庆大霉素+320 U/ml乌司他丁)和C(2 mg/ml庆大霉素+640 U/ml乌司他丁),观察各组细胞增殖能力、乳酸脱氢酶(LDH)水平、细胞凋亡率、ET-1和NO含量、内皮型一氧化氮合酶(e NOS)和诱生型一氧化氮合酶(i NOS)活性以及ET-1、e NOS和i NOS m RNA表达情况。结果与对照组相比,损伤组细胞增殖能力下降,LDH、细胞凋亡率、ET-1、ET-1 m RNA、NO、i NOS和i NOS m RNA表达均增多(P均<0.05)。与损伤组相比,治疗组细胞增殖能力增高,LDH、细胞凋亡率、ET-1、ET-1 m RNA、NO、i NOS和i NOS m RNA表达均减少;这些改变随剂量增加而明显(P均<0.05)。e NOS活性和m RNA表达在5组间的差异无统计学意义(P均>0.05)。结论乌司他丁通过抑制ET-1、NO和i NOS减轻庆大霉素损伤大鼠肾小管上皮细胞,这些作用在一定范围具有剂量依赖性。
Objective To observe the effect of ulinastatin on the expression of endothelin-1 (ET-1) and nitric oxide (NO) in rat renal tubular epithelial cells injured by gentamicin. Methods Rat renal tubular epithelial cells (NRK-52E) were subcultured and divided into control group, injury group (2 mg / ml gentamycin), treatment group A (2mg / ml gentamycin + 160 U / ml (2 mg / ml gentamycin + 320 U / ml ulinastatin) and C (2 mg / ml gentamycin + 640 U / ml ulinastatin) Cell proliferation, lactate dehydrogenase (LDH) level, apoptotic rate, ET-1 and NO content, eNOS and iNOS activity As well as ET-1, eNOS and iNOS m RNA expression. Results Compared with the control group, the proliferative ability of the injured group was decreased, and the expressions of LDH, apoptosis, ET-1, ET-1 m RNA, NO, iNOS and iNOS m RNA were all increased (all P <0.05). Compared with the injury group, the proliferative ability of the treated group increased, and the expression of LDH, apoptosis rate, ET-1, ET-1 m RNA, NO, iNOS and iNOS m RNA were all decreased; (P <0.05). There was no significant difference in eNOS activity and m RNA expression between the 5 groups (all P> 0.05). Conclusion Ulinastatin attenuates gentamicin-induced renal tubular epithelial cells injury through inhibition of ET-1, NO and iNOS in a dose-dependent manner.