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目的：克隆小鼠内皮抑素（endostatin）基因，检测其表达蛋白的生物学活性，应用该蛋白治疗大鼠C6脑胶质瘤。方法：采用RT-PCR法，从小鼠肝组织克隆endostatin基因，重组人pUC19，测序后构建非融合表达载体pBV220-endostatin，使其在DH5α内经温度诱导表达，纯化endostatin蛋白并用鸡胚绒毛膜尿囊膜实验和内皮细胞抑制实验检测其活性，经荷C6胶质瘤大鼠皮下注射该蛋白，检测其抑瘤效应。结果：所获得的endostain基因测序正确，其诱导表达蛋白分子量为20kDa，具有抗血管生成活性；应用该蛋白可抑制荷瘤大鼠C6脑胶质瘤生长。结论：小鼠endostatin基因的成功克隆、表达及应用，为抗血管生成治疗实体瘤研究奠定了实验基础。
OBJECTIVE: To clone the mouse endostatin gene and detect the biological activity of the expressed protein, and apply it to treat C6 glioma in rats. METHODS: RT-PCR was used to clone endostatin gene from mouse liver tissue and recombinant human pUC19. After sequencing, non-fusion expression vector pBV220-endostatin was constructed and induced to express at DH5α temperature. Endostatin protein was purified and chicken embryo chorioallantoic membrane was used. Membrane experiments and endothelial cell inhibition assays were used to test the activity of the protein. Subcutaneous injection of the protein was performed on C6 glioma rats to examine their antitumor effect. RESULTS: The obtained endostain gene was sequenced correctly. The molecular weight of the induced endostain gene was 20 kDa and it had anti-angiogenic activity. The protein could inhibit the growth of C6 brain glioma in tumor-bearing rats. Conclusion: The successful cloning, expression and application of mouse endostatin gene laid an experimental foundation for anti-angiogenesis treatment of solid tumors.