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目的:采用SPE-UPLC法分析养血清脑颗粒中芍药苷的含量。方法:采用C18固相萃取柱去除养血清脑颗粒中的基质,超高效液相色谱法测定芍药苷含量。采用Acquity BEHC18(50 mm×2.1 mm,1.7μm)色谱柱,乙腈-水(25∶75)为流动相,检测波长为230 nm,流速为0.2 mL.min-1,柱温40℃。结果:SPE-UPLC法测得芍药苷浓度在5.0～80.0μg.mL-1范围内与其峰面积线性关系良好(r=0.9999),平均回收率(n=6)为99.4%(RSD=2.83%)。结论:该方法准确、简便并能有效去除药物基质保护色谱柱,可用于该制剂的质量控制。
Objective: To determine the content of paeoniflorin in Yangxue Qingnao Granules by SPE-UPLC. Methods: The matrix of Yangxue Qingnao Granules was removed by C18 solid phase extraction column and the content of paeoniflorin was determined by ultra performance liquid chromatography. The mobile phase was acetonitrile-water (25:75) with a mobile phase of Acquity BEHC18 (50 mm × 2.1 mm, 1.7 μm). The detection wavelength was 230 nm and the flow rate was 0.2 mL.min-1. The column temperature was 40 ℃. Results: The linear relationship between the concentration of paeoniflorin and the peak area in the range of 5.0 ~ 80.0μg.mL-1 was obtained by SPE-UPLC (r = 0.9999) and the average recovery was 99.4% (RSD = 2.83% ). Conclusion: The method is accurate, simple and can effectively remove the drug matrix protection column, which can be used for the quality control of the preparation.