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目的观察不同生长方式的颞骨胆脂瘤上皮和鳞状细胞癌细胞的增殖、凋亡特性,探讨胆脂瘤的发生发展规律。方法于颞骨手术中收集侵袭性胆脂瘤37例和非侵袭性胆脂瘤25 例,鳞状细胞癌8例,颞骨鳞状细胞癌病灶中胆脂瘤上皮5例,正常颞骨皮肤组织8例,分别采用免疫组织化学SP染色技术及末端转移酶介导的原位缺口末端标记染色(TUNEL)技术检测颞骨胆脂瘤上皮及颞骨鳞状细胞癌组织的细胞增殖和凋亡。结果各种类型胆脂瘤上皮细胞增殖指数 (MI)均显著低于颞骨鳞状细胞癌而高于正常表皮;凋亡指数(AI)显著高于正常表皮及颗骨鳞状细胞癌;增殖、凋亡指数比(MI／AI)显著高于颞骨鳞状细胞癌细胞,而与正常表皮差异无统计学意义 (P>0．05)。不同类型胆脂瘤上皮的增殖凋亡指数及增殖凋亡指数相比差异无统计学意义(P> 0．05)。结论颞骨胆脂瘤上皮细胞的生物学特性与颞骨鳞状细胞癌有显著区别,颞骨胆脂瘤上皮细胞的过度增殖和凋亡起着互相调控的作用,但同时也导致了胆脂瘤体积的增大及病变的发展。
Objective To observe the proliferation and apoptosis of epithelial and squamous cell carcinoma of temporal bone cholesteatoma in different growth modes and to explore the occurrence and development of cholesteatoma. Methods In the temporal bone surgery, 37 cases of invasive cholesteatoma and 25 cases of non-invasive cholesteatoma, 8 cases of squamous cell carcinoma, 5 cases of cholesteatoma epithelium in temporal bone squamous cell carcinoma and 8 cases of normal temporal bone tissue The proliferation and apoptosis of epithelial and temporal bone squamous cell carcinoma of the temporal bone were detected by immunohistochemical SP staining and terminal transferase-mediated nick end labeling (TUNEL). Results The proliferation index (MI) of various types of cholesteatoma epithelium was significantly lower than that of temporal bone squamous cell carcinoma and higher than that of normal epidermis. The AI of apoptosis was significantly higher than that of normal epidermis and squamous cell carcinoma. Proliferation, The ratio of apoptotic index (MI / AI) was significantly higher than that of temporal bone squamous cell carcinoma, but there was no significant difference with normal epidermis (P> 0.05). There was no significant difference in proliferative apoptosis index and proliferation and apoptosis index between different types of cholesteatoma epithelium (P> 0.05). Conclusion The biological characteristics of temporal bone cholesteatoma epithelial cells are significantly different from those of temporal bone squamous cell carcinoma. The excessive proliferation and apoptosis of temporal bone cholesteatoma epithelial cells play a role of mutual regulation, but at the same time, the volume of cholesteatoma Increase and pathological development.