Potential control of horseradish peroxidase immobilization on gold electrode

• 作 者: GE Cunwang YU Wei LI Changan W
• 机 构: KeyLaboratoryofMolecularandBiomolecularElectronics（SoutheastUniversity）,DepartmentofChemistry
• 出 处: 中国科学：化学英文版
• 发表日期: 2004年2期
• 关键词: electric POTENTIAL control HORSERADISH peroxidase immobilization gold electro
• 基金项目: This work was supported by the National Natural Science Foundation of China (Grant Nos. 60171005, 60121101), the Natural Science Foundation of Jiangsu Province, China (Grant No. 03KJD310177).

A new approach based on potential control was firstly used for the immobilization of horseradish peroxidase (HRP) as the model protein. The self-assembly monolayer (SAM) was prepared with 2-aminoethanethiol (AET) on the gold electrode. The charge on HRP w