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目的探索倍比稀释虎红平板凝集试验(RBPT)作为布鲁氏菌病(布病)诊断实验的可能性。方法选择2013年内蒙古自治区布病患者血清93份,同时进行试管凝集试验(SAT)和倍比稀释RBPT检测,根据二者实验结果的相关性,以SAT结果作为判定标准,分析不同稀释倍数的RBPT作为诊断截断值的灵敏度和特异度来评价诊断的准确性。结果倍比稀释RBPT受试者工作特征曲线下面积为0.946,以1∶2为截断值时约登指数最高为0.893,灵敏度为89.30%,特异度为100%;以1∶4作为截断值,RBPT的灵敏度为63.10%,约登指数为0.631,特异度为100%;以1∶8作为截断值,RBPT的灵敏度为57.14%,约登指数为0.571,特异度为100%;以1∶16作为截断值,RBPT的灵敏度为19.05%,约登指数为0.191,特异度为100%。结论倍比稀释RBPT以1∶2为截断值时诊断的准确性最高,可以为布病诊断提供参考。
Objective To explore the possibility of using RBPT as a diagnostic test for brucellosis (brucellosis). Methods Ninety-three sera from patients with brucellosis in Inner Mongolia Autonomous Region in 2013 were selected, and the test tube agglutination test (SAT) and the dilution ratio RBPT test were used. According to the correlation between the two test results and SAT test results, the RBPT The diagnostic accuracy is assessed as the sensitivity and specificity of the diagnostic cutoff. Results The area under the working characteristic curve of RBPT subjects with multiple dilution ratio was 0.946. When the cut-off value was 1: 2, the maximum Youton index was 0.893, the sensitivity was 89.30% and the specificity was 100%. The cut-off value of 1: The sensitivity of RBPT was 63.10%, the Youden index was 0.631 and the specificity was 100%. The sensitivity of RBPT was 57.14% with a cut-off value of 1: 8, the Youden index was 0.571 and the specificity was 100% As a cutoff, the RBPT sensitivity was 19.05%, the Youden index was 0.191, and the specificity was 100%. Conclusion The RBPT with 1: 2 ratio of prime dilution ratio has the highest diagnostic accuracy, which can provide a reference for the diagnosis of brucellosis.