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建立一种 HPL C法分离和检测人血浆中吡柔比星的分析方法学。以柔红霉素为内标 ,1ml血浆加0 .1ml(0 .4mol/ L,p H=9.0 )的氯化铵缓冲液碱化后以 CHCl3∶ CH3OH(2∶ 1)混合溶剂提取 2次。在Phenomenex(C1 8)柱上 ,以 0 .0 4mol/ L KH2 PO4(p H=3.0 )∶ CH3CN=6 9∶ 32为流动相 ,荧光检测波长 480 /5 5 0 nm(Ex/ Em)。结果 ,血浆中吡柔比星的线性范围 5～ 10 0 0 μg/ L(r=0 .997) ,方法的回收率为 95 .3% ,日内及日间变异性 RSD均小于 5 %。结论 :本方法适用于临床吡柔比星的血药浓度监测和药代动力学研究。
To establish an analytical method for the separation and detection of pirarubicin in human plasma by HPL-C method. To daunorubicin as an internal standard, 1ml of plasma plus 0.1ml (0.4mol / L, p H = 9.0) of ammonium chloride alkalinization and CHCl3: CH3OH (2: 1) mixed solvent extracted twice . The fluorescence detection wavelength was 480/5050 nm (Ex / Em) on a Phenomenex (C1 8) column with 0.040 mol / L KH2PO4 (p H = 3.0): CH3CN = 6 9:32 as mobile phase. As a result, the linear range of pirarubicin in plasma ranged from 5 to 10 0 μg / L (r = 0.997) and the recovery rate was 95.3%. The intra-day and inter-day variability RSDs were less than 5%. Conclusion: This method is suitable for the clinical monitoring and pharmacokinetic study of pirarubicin.