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以北海道黄杨(Euonymus japonicus‘Cuzhi’)下胚轴为外植体进行离体培养,以MS和B5为基本培养基,附加不同浓度的细胞分裂素(6-BA,KT)及生长素(NAA,IBA)诱导下胚轴直接再生不定芽。结果表明,不定芽未经过愈伤组织而直接产生于下胚轴的表皮或近表皮等表层细胞;不同的激素浓度和组合以及不同的培养基对不定芽的分化有影响;下胚轴的不同部位不定芽的分化能力差异显著。适宜不定芽分化的最佳培养体系为MS+1.5mg·L-16-BA+0.05mg·L-1NAA,最佳外植体为靠近子叶端的下胚轴部分,其分化率最高达63.64%;不定芽增殖培养基为MS+2.0mg·L-16-BA+0.2mg·L-1NAA,增殖系数为3～5;1/2MS+1.0mg·L-1IBA+100mg·L-1活性炭适于再生幼苗的生根,生根苗经移栽成活。
Hypocotyls of Euonymus japonicus ’Cuzhi’ were used as explants for in vitro culture. MS and B5 were used as basal medium supplemented with different concentrations of 6-BA and KT and NAA , IBA) induced direct regeneration of adventitious buds in hypocotyls. The results showed that the adventitious buds were directly produced on the epidermis or epidermis cells of epicyte without callus. Different hormones concentration and combination and different media had influence on the adventitious bud differentiation. The hypocotyls differed Adventitious buds differentiated significantly. The best culture system suitable for adventitious bud differentiation was MS + 1.5 mg · L-16-BA + 0.05 mg · L-1 NAA. The best explant was hypocotyl near cotyledon end, with the highest rate of 63.64%. The multiplication medium of adventitious buds was MS + 2.0mg · L-16-BA + 0.2mg · L-1NAA with multiplication coefficient of 3 ~ 5; 1/2 MS + 1.0mg · L -1 IBA + 100mg · L -1 activated carbon was suitable Regeneration seedling rooting, rooting by transplanting alive.