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目前国内外比较广泛采用的测定昆虫拒食剂拒食活性的方法主要有圆叶片法及改进的圆叶片法、体重法、排泄物法及电讯号法,现分述如下。 一、圆叶片法及改进的圆叶片法 如果以食叶量较大的咀嚼式口器昆虫为试虫,则可采用圆叶片法测定拒食活性。将供试样品用丙酮定溶至预定浓度(如样品难溶于丙酮,则可先用少量其它溶剂如乙醇、二甲亚砜、乙醚等溶解,再以丙酮稀释至预定浓度)。将试虫喜食的叶
Currently widely used to determine the antifeedant insect fasting antifeedant methods mainly round leaf method and improved round leaf method, body weight method, fecal method and the telecommunications method, are as follows. A round blade method and the improved round blade method If the larger amount of chewing insects mouth insects as test insects, you can use the method of determination of anti-leaf activity round. If the sample is insoluble in acetone, it can be dissolved with a small amount of other solvents such as ethanol, dimethylsulfoxide, diethyl ether and the like, and then diluted to a predetermined concentration with acetone. Test insects eat leaves