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The transition dipole moments (TDMs) of ultracold 85 Rb133 Cs molecules between the lowest vibrational ground level,X1 Σ+ (v =0,J =1),and the two excited rovibrational levels,23Πo+ (v =10,J =2) and 21Π1 (v =22,J =2),are measured using depletion spectroscopy.The ground-state 85Rb133Cs molecules are formed from cold mixed component atoms via the 23Π0-(v =11,J =0) short-range level,then detected by time-of-flight mass spectrum.A home-made exteal-cavity diode laser is used as the depletion laser to couple the ground level and the two excited levels.Based on the depletion spectroscopy,the corresponding TDMs are then derived to be 3.5(2)× 10-3ea0 and 1.6(1)×10-2eao,respectively,where eao represents the atomic unit of electric dipole moment.The enhance of TDM with nearly a factor of 5 for the 21 Π1 (v =22,J =2) excited level means that it has stronger coupling with the ground level.It is meaningful to find more levels with much more strong coupling strength by the represented depletion spectroscopy to realize direct stimulated Raman adiabatic passage transfer from scattering atomic states to deeply molecular states.