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本研究旨在观察不同时程模拟微重力效应下大鼠脑组织氧化应激的变化,为揭示模拟微重力效应下神经系统氧化应激的发生机制和开发相应的药物防护提供理论基础。将40只成年雄性Sprague-Dawley(SD)大鼠按体重配对原则随机分为对照组及不同时间(分别处理7,14,21,28d)尾悬吊模拟微重力效应组,应用Griess法、硫代巴比妥酸反应物法(thiobarbituric acid reactive substance assay,TBARS)、ELISA法和铁离子还原法(ferric reducing ability of plasma,FRAP)分别检测大鼠小脑、大脑皮层和海马组织内的活性氮(reactive nitrogen species,RNS)、丙二醛(malondialdehyde,MDA)、硝基酪氨酸(nitrotyrosine,NT)和总抗氧化能力(total antioxidant capacity,TAC)。结果显示,与对照组相比:(1)小脑组织在尾悬吊7d后NT含量明显升高,之后降低,28d再次升高;14d后MDA含量明显增加;21d后RNS含量显著升高,TAC显著降低;(2)大脑皮层在尾悬吊14d后NT含量显著升高;21d后MDA含量明显升高,TAC明显降低;(3)海马组织在尾悬吊7d后RNS含量明显升高,之后降低,28d后再次升高;21d后MDA含量明显升高;28d后NT含量显著上升;7d后TAC明显升高,持续至14d,然后恢复。上述结果表明,模拟微重力效应使得大鼠脑组织发生了氧化应激,不同部位脑组织对氧化应激的反应程度不同;在对不同时程模拟微重力效应的响应过程中,大鼠脑组织呈现了从适应性反应到不可逆性损伤的变化历程。
The purpose of this study was to observe the changes of oxidative stress in brain tissue under different simulated microgravity effects in different time courses and to provide a theoretical basis for revealing the mechanism of neuro-oxidative stress under simulated microgravity effect and developing corresponding drug protection. Forty adult male Sprague-Dawley (SD) rats were randomly divided into control group and tail-suspended simulated microgravity effect groups at different times (7, 14, 21 and 28 days) TBARS, ELISA and ferric reducing ability of plasma (FRAP) were used to detect the activity of reactive nitrogen (NOS) in the cerebellum, cerebral cortex and hippocampus reactive nitrogen species (RNS), malondialdehyde (MDA), nitrotyrosine (NT) and total antioxidant capacity (TAC). The results showed that compared with the control group, the content of NT in cerebellum increased significantly on the 7th day after tail suspension, and then decreased on the 28th day. The content of MDA increased significantly on the 14th day, and the content of RNS increased significantly on the 21st day (2) The content of NT in cerebral cortex increased significantly after 14 days of tail suspension, and the content of MDA increased significantly and the TAC decreased significantly after 21 days. (3) The content of RNS in hippocampus increased significantly after 7 days, And then increased again 28 days later. After 21 days, the content of MDA increased significantly. After 28 days, the content of NT increased significantly. After 7 days, the TAC increased obviously and continued until 14 days. The above results show that simulated microgravity effect causes oxidative stress in rat brain tissue and different degrees of brain tissue response to oxidative stress. In response to simulated microgravity effects at different time points, rat brain tissue Presented from the adaptive response to the irreversible damage changes course.