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试验以健康的建鲤为试验动物,研究在其日粮中添加不同水平的酵母甘露寡糖对其生产性能的影响,确定酵母甘露寡糖在鲤鱼中的添加水平,并对其作用机制进行了初步探讨。选用健康均匀的鲤鱼随机分为6组,分别使用不同的饵料,按正常的饲养管理程序进行。1组为对照组,饲喂基础日粮,2、3、4、5、6组为试验组,分别在基础日粮中添加0.05%、0.1%、0.2%、0.3%、0.4%的酵母甘露寡糖(商品名为奥奇素,Bio-MOS),试验期为45d,以测定甘露寡糖对鲤鱼血液生化指标的影响。结果表明,血清中SOD分别比对照组高6、9、10、23、27IU,5、6组与对照组之间差异极显著(P<0.01);血液中钾、钙、镁、氯离子的含量随MOS添加量升高有所上升,但是效果不显著(P>0.05);血液中的钙随MOS的添加增加明显(P<0.01);血液中总蛋白、白蛋白、球蛋白含量随MOS添加而升高:其中试验组3、4、5、6组的总蛋白比对照组高3.19g/L(P<0.01)、4.07g/L(P<0.01)、2.16g/L(P<0.05)、1.21g/L(P<0.05),试验3、4、5、6组的白蛋白比对照组高1.18g/L(P<0.01)、1.69g/L(P<0.01)、1.42g/L(P<0.01)、1.11g/L(P<0.01),其余各组与对照组相比无显著差异,试验3和4组的球蛋白比对照组高2.01g/L(P<0.01)、1.38g/L(P<0.01),其余各组与对照组相比无显著差异;随添加量的增加红细胞数量和血红蛋白量有升高的趋势,4、5、6组与对照组相比差异极显著。
In this study, healthy Jian carp was used as experimental animal to study the effects of adding different levels of mannan oligosaccharides to their diet on the performance of carp, and to determine the added level of mannan oligosaccharides in carp, and its mechanism of action Preliminary discussion. Choose healthy and uniform carp were randomly divided into 6 groups, respectively, using different bait, according to the normal feeding and management procedures. 1 group as the control group, fed the basal diet, 2,3,4,5,6 group as the experimental group, were added to the basic diet 0.05%, 0.1%, 0.2%, 0.3%, 0.4% yeast mannose Oligosaccharides (brand name Odotin, Bio-MOS), the test period was 45d, to determine the effect of mannooligosaccharides on carp blood biochemical indicators. The results showed that the SOD in serum was 6, 9, 10, 23 and 27 IU higher than that in the control group, and the difference between the 5 and 6 groups was significant (P <0.01). The contents of potassium, calcium, magnesium and chloride (P <0.01). The content of total protein, albumin and globulin in blood increased with the increase of MOS content, but the effect was not significant (P> 0.05) (P <0.01), 4.07g / L (P <0.01), 2.16g / L (P <0.01) (P <0.01), 1.69g / L (P <0.01), 1.42g / L (P <0.01), 1.21g / L g / L (P <0.01), 1.11g / L (P <0.01). There was no significant difference between the other groups and the control group. The globulin in groups 3 and 4 was 2.01g / L higher than the control group (P < 0.01) and 1.38g / L (P <0.01). There was no significant difference between the other groups and the control group. The red blood cell count and the amount of hemoglobin tended to increase with the increase of the dosage, The difference was significant.