论文部分内容阅读
目的:观察组蛋白去乙酰化酶抑制剂(HDACIs)丁酸钠(NaB)及曲妥珠单抗Trastuzumab对人乳腺癌细胞株SKBR3细胞增殖、细胞周期及细胞凋亡的影响,探讨NaB及Trastuzumab调控乳腺癌细胞增殖的分子机制。方法:乳腺癌SKBR3细胞经NaB、Trastuzumab单独或联合作用后,MTT法检测细胞增殖情况,流式细胞仪分析细胞周期分布及细胞凋亡,Western Blot方法检测p27Kip1的表达。结果:NaB单独用药显著抑制SKBR3细胞增殖,促进细胞G0/G1期阻滞,增加细胞凋亡和p27Kip1蛋白的表达,P<0.05;20μg/mL Trastuzumab单独用药,对细胞有增殖抑制和细胞周期阻滞作用(P<0.05),但对细胞凋亡及p27Kip1蛋白表达无明显影响,P>0.05。Trastuzumab可协助NaB增加对SKBR3的抗肿瘤作用及p27Kip1蛋白表达,P<0.05。结论:Trastuzumab联合NaB抑制乳腺癌细胞的增殖,促进细胞周期阻滞及细胞凋亡的发生,以上过程可能是通过增加p27Kip1的蛋白表达来实现。
OBJECTIVE: To observe the effects of sodium butyrate (NaACb) and trastuzumab on the proliferation, cell cycle and apoptosis of human breast cancer cell line SKBR3 and to explore the effects of NaB and Trastuzumab Regulate the molecular mechanism of breast cancer cell proliferation. Methods: SKBR3 cells were treated with NaB or Trastuzumab alone or in combination. MTT assay was used to detect cell proliferation. Cell cycle distribution and apoptosis were analyzed by flow cytometry. The expression of p27Kip1 was detected by Western Blot. Results: NaB alone significantly inhibited the proliferation of SKBR3 cells, promoted the arrest of G0 / G1 phase and increased the apoptosis and expression of p27Kip1 protein, P <0.05. Trastuzumab alone could inhibit the proliferation of cells and the cell cycle resistance (P <0.05), but had no significant effect on apoptosis and p27Kip1 protein expression (P> 0.05). Trastuzumab can help NaB increase anti-tumor effect of SKBR3 and p27Kip1 protein expression, P <0.05. Conclusion: Trastuzumab combined with NaB can inhibit the proliferation of breast cancer cells and promote cell cycle arrest and apoptosis. The above process may be through increasing the protein expression of p27Kip1.