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目的:探讨慢性髓性白血病(CML)和急性淋巴细胞白血病(ALL)细胞中Wnt信号通路活性是否存在差异。方法:利用慢病毒基因转染法在经BCR-ABL融合基因诱导形成的CML和ALL细胞中建立Wnt信号通路报告系统BAT-RED,检测2种白血病细胞中Wnt信号通路活性;PCR方法检测2种细胞中BAT-RED基因整合情况;通过向培养细胞中添加氯化锂,人为增强Wnt信号活性,反向验证Wnt信号通路活性在2种细胞中存在差异。结果:PCR结果显示2种白血病细胞都稳定整合了Wnt信号通路报告基因BAT-RED,在42%(188/448)的慢性粒细胞白血病细胞中可以检测到Wnt信号通路被激活,而ALL细胞仅散在少数细胞中可以检测到Wnt信号通路报告基因BAT-RED被激活;添加氯化锂后,ALL细胞中Wnt信号通路活性增强。结论:CML细胞中Wnt通路活性明显高于ALL细胞,提示Wnt信号通路在2种类型白血病细胞的增殖和演进过程中具有不同作用。
Objective: To investigate whether there is difference in Wnt signaling pathway between chronic myelogenous leukemia (CML) and acute lymphoblastic leukemia (ALL). Methods: Wnt signaling system BAT-RED was established in CML and ALL cells induced by BCR-ABL fusion gene by lentiviral gene transfection method. The activity of Wnt signaling in 2 leukemia cells was detected. Cell integration of BAT-RED gene; by adding lithium chloride to cultured cells, artificially enhance Wnt signal activity, reverse verification Wnt signaling pathway activity in the two kinds of cells in the difference. Results: The results of PCR showed that Wnt signaling pathway gene BAT-RED was stably integrated in both leukemia cells and Wnt signaling pathway was detected in 42% (188/448) of chronic myeloid leukemia cells, whereas ALL cells only Scattered in a small number of cells can be detected Wnt signaling pathway gene BAT-RED is activated; adding lithium chloride, ALL cells Wnt signaling pathway activity increased. CONCLUSION: Wnt pathway activity in CML cells is significantly higher than that in ALL cells, suggesting that Wnt signaling pathway plays different roles in the proliferation and evolution of the two types of leukemia cells.