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本文通过对健康成年wistar大鼠饲以鹿茸乙醇提取液,分批在用药1个月、2个月、3个月后处死,光镜下观察用药后大鼠睾丸生精细胞和睾丸间质细胞的形态特点;并采用体视学方法计量间质细胞的体密度(Vv),数密度(Nv),平均自由程(πλ),表面积密度(Sv);测算了生精细胞的层数,每个曲精管断面精原细胞数及其细胞核分裂率。结果发现:饲饮药物1个月即可促使大鼠生精细胞核分裂率升高(P<0.01);饲药2个月,不但大鼠生精细胞核分裂率升高而且每个曲精管断面的精原细胞数量也增多(P<0.01);饲药3个月大鼠除精原细胞核分裂率升高、精原细胞数/曲精管增多外,生精细胞的层数亦明显增多。体视学计量结果表明饲药3个月大鼠睾丸间质细胞Nv、λ有显著差异(P<0.01);提示鹿茸乙醇提取液饲用1个月后对正常成年大鼠生精过程有一定程度促进作用,随饮用时间延长,作用愈趋显著。
In this paper, healthy adult wistar rats were fed with antler ethanol extract, and were sacrificed in batches 1 month, 2 months and 3 months after treatment. The spermatogenic cells and testicular stromal cells of the testes were observed under light microscope. Morphological characteristics; and using stereological methods to measure the interstitial cell volume density (Vv), number density (Nv), mean free path (πλ), surface area density (Sv); measured the number of layers of spermatogenic cells, each The number of spermatogonia in the seminiferous tubules and their cell division rate. The results showed that dietary drugs could promote the nuclear fission rate of spermatogenic cells in rats for 1 month (P<0.01). For 2 months, not only the nuclear fission rate of rat spermatogenic cells increased, but also each semen The number of spermatogonial cells in the tube section also increased (P<0.01); the number of spermatogenic cells in the rats at 3 months after feeding was higher than that in the spermatogonia, and the number of spermatogonia and the number of seminiferous tubules increased. Also significantly increased. The results of stereological measurement showed that the Nv and λ of testicular stromal cells of rats fed for 3 months had significant differences (P<0.01), suggesting that the ethanol extract of pilose antler was used for spermatogenesis of normal adult rats after 1 month of feeding. It has a certain degree of promoting effect. With the prolonged drinking time, the effect becomes more pronounced.