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目前在药物工业中,高效液相色谱法在通常条件下不需制备成衍生物,即可分析复杂、不挥发和不稳定的化合物,比气液和薄层色谱法所需的净化样品量少。进样可用注射器或试样阀。当进样体积相当大且多次注射时,可选用进样阀。此法重现性较好。注射器可用来注射极小量的试样提取液。通常应用的是固定波长和可变波长的紫外检测器及折射率检测器。可变波长检测器能使探测化合物具有更高的灵敏度和选择性。折射率检测器是常用的检测器,但灵敏度差,除非将温度小心地控制恒温。负峰说明它很难与电子积分仪联用。
Currently in the pharmaceutical industry, HPLC does not require the preparation of derivatives under the usual conditions for the analysis of complex, non-volatile and labile compounds, as compared to less purified samples required for gas-liquid and thin-layer chromatography . Injectable syringe or sample valve available. When the injection volume is quite large and multiple injections, the injection valve can be used. This method reproducibility is better. Syringes can be used to inject small amounts of sample extract. Commonly used are fixed wavelength and variable wavelength UV detector and refractive index detector. Variable wavelength detectors make probe compounds more sensitive and selective. Refractive index detector is a commonly used detector, but the sensitivity is poor, unless the temperature is carefully controlled thermostat. The negative peak shows that it is difficult to use with the electronic integrator.