Optimization of Electroporation Parameters for Immature Embryos of indica Rice （ Oryza sativa）

• 作 者: REN Yu-jun ZHAO Jie
• 机 构: KeyLaboratoryoftheMinistryofEducationforPlantDevelopmentalBiology
• 出 处: 水稻科学：英文版
• 发表日期: 2008年1期
• 关键词: 电穿孔 稻子 胚芽 瞬变现象 electroporation β-glucuronidase gene immature embryo indica ri
• 基金项目: ACKNOWLEDGEMENTSThis project was supported by the National Natural Science Foundation of China （Grant No. 30570103） and the Key Grant Project of Chinese Ministry of Education （Grant No. 307018）. We th

To obtain a suitable condition for electroporation transformation in indica rice, the 10-day-old immature embryos were selected for optimization experiments. The results showed that one pulse at 850 V/cm, 950 μF capacitance, 200 μL electroporation buffer