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背景:雌激素治疗绝经后骨质疏松症产生的不良反应较大。目的:观察小剂量葛根素联合雌二醇对去卵巢大鼠骨组织的影响。方法:120只健康雌性大白鼠等分成只切除卵巢旁的一小块脂肪垫的假手术组和切除双侧卵巢的去卵巢模型组、葛根素组、雌二醇组及葛根素+雌二醇组。1周后葛根素组、雌二醇组和雌二醇+葛根素组分别皮下注射葛根素(50mg/kg,1次/d)、雌二醇(200μg/kg,2次/周)和雌二醇(100μg/kg,2次/周)+葛根素(25mg/kg,1次/d)。结果与结论:去卵巢模型组大鼠骨组织稀疏,骨钙、磷水平和骨密度明显低于假手术组(P<0.01),而经雌二醇和/或葛根素治疗后大鼠骨组织形态明显改善、骨钙、磷水平和骨密度明显升高。其中小剂量的葛根素联合雌二醇治疗与较大剂量的葛根素或雌二醇治疗效果接近。提示较小剂量的雌二醇与葛根素联合治疗也可对去卵巢大鼠骨质疏松症产生良好的治疗效果。
Background: Estrogen treats postmenopausal osteoporosis with greater adverse reactions. Objective: To observe the effects of low-dose puerarin combined with estradiol on the bone tissue of ovariectomized rats. Methods: 120 healthy female rats were equally divided into two groups: the sham operation group and the ovariectomized ovariectomized group, puerarin group, estradiol group and puerarin + estradiol group. After 1 week, Puerarin, estradiol and estradiol + puerarin groups were subcutaneously injected with puerarin (50mg / kg, once daily) and estradiol (200μg / kg twice weekly) Diol (100 μg / kg, 2 times / week) + Puerarin (25 mg / kg, 1 time / d). RESULTS AND CONCLUSION: In ovariectomized rats, the bone tissue was sparse, with the levels of bone calcium, phosphorus and bone mineral density being significantly lower than those in the sham operation group (P <0.01). However, the bone morphological changes of rats after estradiol and / or puerarin treatment Significant improvement, bone calcium, phosphorus levels and bone mineral density increased significantly. Small doses of puerarin combined with estradiol treatment and higher doses of puerarin or estradiol treatment effect close. Tip Lower doses of estradiol and puerarin combined treatment of ovariectomized rats can also produce a good therapeutic effect of osteoporosis.