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目的：建立用于临床的环孢素Ａ的放射受体测定法，并与荧光偏振免疫测定法进行比较。方法：利用亲环素Ａ（ＣｙＰＡ）能在体外与环孢素Ａ（ＣｓＡ）高效结合的特性，以重组人亲环素Ａ（ｒｈＣｙＰＡ）作为特异性的结合蛋白，３Ｈ－ＣｓＡ作为示踪剂，建立ＣｓＡ的放射受体测定法。结果：方法的有效测定范围为４３．８～２８００μｇ／Ｌ，灵敏度为４３．８μｇ／Ｌ，批内变异系数为８．２％（ｎ＝８），批间变异系数为１０．６％（ｎ＝８），标本提取与测定方法的全程回收率分别为１０４．２％和１０６．３％；对ＣｓＡ及其几种类似物与ｒｈＣｙＰＡ的亲和力进行比较，以ＣｓＡ与ｒｈＣｙＰＡ的亲和力为最佳；在实际应用中与荧光偏振免疫测定法进行比较（ｎ＝３０），二者测值高度相关，ｒ为０．９６４２，回归方程为ｙ＝３９．１１２＋０．９９ｘ。结论：该方法具有灵敏可靠，特异性、重复性、稳定性和经济性好等优点，适用于ＣｓＡ全血浓度的临床测定。
OBJECTIVE: To establish a radioreceptor assay for clinical cyclosporin A and to compare with fluorescence polarization immunoassay. METHODS: Cycl was used to efficiently bind CsA in vitro. Recombinant human cyclophilin A (rhCyPA) was used as a specific binding protein and 3H-CsA as a tracer , Establishing a radioisotope assay for CsA. Results: The effective range was 43.8 ~ 2800μg / L, the sensitivity was 43.8μg / L, the intra-assay CV was 8.2% (n = 8), the inter-assay CV was 10.6% = 8). The recovery rate of the method of sample extraction and determination was 104.2% and 106.3%, respectively. The affinity of CsA and its analogues with rhCyPA was compared, and the affinity of CsA and rhCyPA was the best. In practical application, compared with fluorescence polarization immunoassay (n = 30), the correlation between them was highly correlated, r was 0.9642, and the regression equation was y = 39.112 + 0.99x. Conclusion: The method is sensitive, specific, reproducible, stable and economical. It is suitable for the clinical determination of CsA whole blood concentration.