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简要叙述了核磁共振技术(NMR)在蛋白质领域的研究及应用。NMR法通过测定蛋白质在稀溶液状态下反应位点的特定参数来计算蛋白质的三级结构,并可深入了解一定时间范围内化学反应和蛋白质构象转变的动力学过程。通过NMR对抗原决定簇和抗体CDR作图,可以分析其一级结构和三维构象;对抗原抗体动力学的分析,对于设计基因疫苗、检测细胞表面抗原提呈以及分析抗原抗体复合物的构象变化也有着重要意义。
Briefly describe the research and application of nuclear magnetic resonance (NMR) in the field of protein. The NMR method calculates the tertiary structure of a protein by measuring the specific parameters of the protein’s reaction site in a dilute solution and provides insights into the kinetics of chemical reactions and protein conformational transitions over time. The primary structure and the three-dimensional conformation can be analyzed by NMR mapping of the epitopes and the CDRs of the antibody; the analysis of the antigen-antibody kinetics can be useful for designing a gene vaccine, detecting cell surface antigen presentation and analyzing the conformational changes of the antigen-antibody complex It is also significant.