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目的探讨维生素Ｃ（Ｖｉｔ ．Ｃ） 对培养在不同氧浓度气体环境中的家兔肺泡巨噬细胞（ＡＭ） 受激发光功能的影响。方法利用一台可以检测细胞发光的恒温装置培养ＡＭ，在连续通气条件下用发光仪记录ＰＭＡ（ 弗波豆蔻乙酸） 激发的ＡＭ 化学发光水平。结果培养在０ ．５ ％ 氧环境５ ｈ 的ＡＭ 存活率和发光水平分别为暴露前的５０ ％ 和４５ ％ ，并随培养介质中添加的Ｖｉｔ．Ｃ 剂量增加而逐步下降。提高气体氧浓度有利于增强细胞活力，并可部分抵消由Ｖｉｔ．Ｃ 引起的ＡＭ 受激发光水平降低。结论含氧气体有利于培养细胞的受激发光功能和细胞存活；给培养介质添加高浓度Ｖｉｔ ．Ｃ 对ＡＭ 有明显毒害作用。
Objective To investigate the effect of vitamin C on the stimulated luminescence of alveolar macrophages (AMs) cultured in air with different oxygen concentrations. METHODS AM was cultured using a thermostatic device that detects the luminescence of cells and the AM chemiluminescence level excited by PMA (phorbol myristic acid) was recorded using a luminometer under continuous aeration. Results were cultured at 0. The survival rate and the luminescence level of AM at 5% oxygen environment for 5 h were 50% and 45% before exposure, respectively, and with the addition of Vit in the culture medium. C dose increased gradually decreased. Increasing the oxygen concentration of the gas helps to increase the cell viability and partially offset by Vit. C-induced AM stimulated emission levels decreased. CONCLUSION: Oxygen-containing gas is beneficial to the stimulated luminescence and cell survival of cultured cells. High concentrations of Vit were added to the culture medium. C has a significant toxic effect on AM.