论文部分内容阅读
本文报道了一种分离脑内血管紧张素Ⅱ结合位点的受体放射分析法。~3H-血管紧张素Ⅱ在37℃与大鼠皮层下脑组织膜受体结合是可饱和的,饱和点大约在配基浓度7nM,最大结合能力(Bmax)是104fmol/mg(蛋白),平衡解离常数(KD)是5.9nM,在KD浓度时特异结合是总结合的72.2％,并且是可逆的。孵育30分钟后,特异结合基本达到平衡稳定状态。证明大鼠皮层下突触膜有血管紧张素大鼠脑Ⅱ的高亲和力结合部位,这可能就是血管紧张素受体存在的部位。
This paper reports a receptor radiometric assay for the isolation of angiotensin II binding sites in the brain. ~ 3H-Angiotensin II is saturated at 37 ° C and binds to rat subepithelial membrane receptors with a saturation point of approximately 7 nM at the ligand concentration and 104 fmol / mg (protein) at the maximal binding capacity (Bmax) The dissociation constant (KD) is 5.9 nM, and the specific binding at KD concentration is 72.2% of the total binding and is reversible. After 30 minutes of incubation, the specific binding reached the equilibrium state basically. Proved that the rat subepithelial synaptic membrane angiotensin Ⅱ rat brain with high affinity binding sites, which may be where the presence of angiotensin receptors.