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为明确小麦(Triticum aestivum)-柔软滨麦草(Leymus mollis(Trin.)Hara)易位系M8657-1的抗条锈性,用中国小麦条锈菌(Puccinia striiform f.sp.stritici)流行小种条中30号、条中31号、水源11-4和水源11-11生理小种,对M8657-1和铭贤169的杂交后代进行苗期抗条锈性遗传分析。结果表明,易位系M8657-1对条中30号和水源11-11的抗条锈性均1对隐性核基因控制;对条中31号的抗条锈性由2对显性核基因(互补作用)控制;对水源11-4的抗条锈性由1对显性核基因控制。将控制水源11-4抗病性的基因暂时命名为YrElm1-4,以接种水源11-4的F2正交群体为研究对象,应用BSA法进行了SSR分析。从320对SSR引物组合中筛选到3个与主效抗病基因YrElm1-4连锁的多态性微卫星标记,它们分别是Xgwm636、Xwmc522和Xwmc453,根据3个微卫星标记位点的染色体位置,推出YrElm1-4位于小麦2AS染色体上,这3个标记可用于分子标记辅助育种。
In order to understand the stripe rust resistance of Triticum aestivum-Leymus mollis (Trin.) Hara translocation line M8657-1, we used the genotypes of Puccinia striiform f. Sp. Tritici Stripe rust resistance of seedlings of hybrid progeny of M8657-1 and Mingxian169 was analyzed in the article 30, Article 31, water source 11-4 and water source 11-11. The results showed that the resistance to stripe rust was controlled by one translocation line M8657-1 against the stripe rust in 30 and 11-11, respectively. The stripe rust resistance of stripe number 31 was controlled by two pairs of dominant nuclear genes (Complementarity) control; stripe rust resistance of water source 11-4 by a pair of dominant nuclear gene control. The gene controlling the resistance of water source 11-4 was temporarily named as YrElm1-4. The SSR analysis was performed using the BSA method. Three pairs of microsatellite markers were selected from 320 pairs of SSR primer combinations and linked to major resistance gene YrElm1-4. They were Xgwm636, Xwmc522 and Xwmc453, respectively. Based on the chromosomal location of the three microsatellite markers, Launched YrElm1-4 located on wheat 2AS chromosome, these three markers can be used for molecular marker assisted breeding.