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本文在一个克隆化的基因组系统中,研究了DNA超螺旋结构在α-晶体蛋白基因表达中的调控作用;测定了调节基因区及其对转录过程的影响.结果显示α-晶体蛋白基因可被RF-36蛋白因子特异性激活.足迹法分析表明其调控结合区为核苷酸“-AGGGCTGGAA-”,能与特定的DNA配位体结合,例如抗Z-DNA单克隆抗体和Chiral金属复合物,此种结合表明α-晶体蛋白基因富含“GC”盒,并具有类似“Z”型的DNA构型.所获结果还提示,DNA高度有序的结构在α-晶体蛋白基因的表达调控过程中起重要作用.
In a cloned genomic system, the regulatory effect of DNA supercoiled structures on α-crystallin gene expression was examined, and the effect of regulating gene regions and their effects on transcription was examined. The results show that α-crystallin genes can be RF-36 protein factor. Footprinting analysis showed that its regulatory binding region is a nucleotide “-AGGGCTGGAA-” that binds to specific DNA ligands such as anti-Z-DNA monoclonal antibodies and Chiral metal complexes , This binding indicates that the α-crystallin gene is rich in “GC” boxes and has a DNA configuration resembling “Z.” The results obtained also suggest that the highly ordered structure of DNA is regulated by the expression of α-crystallin genes Play an important role in the process.