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This paper studied on the way of Coleus blumei,the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine(6-BA),α-naphthaleneacetic acid(NAA)and indole-3-butyric acid(IBA).The optimal conditions of callus induction from explants were achieved on the medium containing 6-BA(2.0 mg.L-1)and NAA(1.0 mg.L-1).Shoot tips were induced on the medium containing 6-BA(4.0 mg.L-1)and NAA(0.5 mg.L-1).The same media conditions were found suitable for shoot multiplication,we multiplied shoots rooted best on1/2 MS medium supplemented with IBA(0.1 mg.L-1).
This paper studied on the way of Coleus blumei, the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine (6-BA), α-naphthaleneacetic acid (NAA) and indole-3- butyric acid (IBA) .The optimal conditions of callus induction from explants were achieved on the medium containing 6-BA (2.0 mg.L-1) and NAA (1.0 mg.L-1) .Shoot tips were induced on the medium containing 6-BA (4.0 mg.L-1) and NAA (0.5 mg.L-1). The same media conditions were found suitable for shoot multiplication, we multiplied shoots rooted best on 1/2 MS medium supplemented with IBA (0.1 mg. L-1).