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为进一步开展人白细胞介素Ⅱ（ＩＬ２）基因修饰的肿瘤疫苗的研究，在已构建的人白细胞介素ⅡｃＤＮＡ表达载体（ｐｃＤＮＡ３／ＩＬ）的基础上，利用脂质体，将其引入人肝癌细胞株７７２１，通过Ｇ４１８ 抗性筛选获得阳性克隆，用活细胞计数法检测阳性克隆的细胞培养上清中的ＬＩ２ 活性，结果表明ｐｃＤＮＡ３／ＩＬ２ 转染的７７２１ 细胞所分泌的ＩＬ２ 水平明显高于阳性对照ｐＢｃ１２／ＨＩＶ／ＩＬ２ 转染的７７２１ 细胞所分泌的ＩＬ２ 水平。说明重组表达载体ｐｃＤＮＡ３／ＩＬ２ 的表达效率高于ｐＢｃ１２／ＨＩＶ／ＩＬ２，这为下一步制备新型瘤苗打下了基础。
In order to further develop a study of human interleukin 2 (IL2) gene-modified tumor vaccines, based on the established human interleukin II cDNA expression vector (pcDNA3/IL), using liposomes, it was introduced into humans. Hepatoma cell line 7721 was positive cloned by G418 resistance screening and live cell counting was used to detect LI2 activity in cell culture supernatants of positive clones. The results showed that IL2 secreted by pcDNA3/IL2 transfected 7721 cells. The level was significantly higher than the level of IL2 secreted by the positive control pBc12/HIV/IL2 transfected 7721 cells. This indicated that the expression efficiency of the recombinant expression vector pcDNA3/IL2 was higher than that of pBc12/HIV/IL2, which laid the foundation for the preparation of new tumor vaccine in the next step.