论文部分内容阅读
AIM:To study the expression and serum level of HBxAg,Fas and FasL in tissues of HCC patients,and toassess therelationship between HBxAg and Fas/FasL system.METHODS:Tissues from 50 patients with HCC were testedfor the expression of HBxAg,Fas and FasL by S-Pimmunohistochemistry.Serum levels of sFas/sFasL andHBsAg/HBeAg were measured by ELISA assay.HBV X genewas detected by PCR in serum and confirmed by automaticsequencing.Fifty cases of liver cirrhosis and 30 normalcontrols were involved in serum analysis.RESULTS:The expression of HBxAg,Fas and FasL incarcinoma tissues was 96 %,84 % and 98 %,respectively.Staining of HBxAg,Fas and FasL was observed predominatelyin cytoplasms,no significant difference was found in intensitybetween HBxAg,Fas and FasL (P>0.05).HBxAg,Fas and FasLmight express in the same area of carcinoma tissues and thisco-expression could be found in most patients with HCC.Themean levels of sFas in serum from HCC,cirrhosis and normalcontrols were 762.29±391.56 μg·L~(-1),835.36±407.33 μg·L~(-1) and238.27±135.29 μg·L~(-1).The mean levels of sFasL in serumfrom HCC,cirrhosis and normal controls were 156.36±9.61μg·L~(-1),173.63±18.74 μg·L~(-1) and 121.96±7.83 μg·L~(-1).Statistical analysis showed that both sFas and sFasL in HCCand cirrhosis patients were significantly higher than thosein normal controls (P<0.01).Serum HBV X gene was foundin 32 % of HCC patients and 46 % of cirrhotic patients.There was no significant relationship between serum levelof sFas/sFasL and serum X gene detection (P>0.05).Eightpercent of HCC patients with negative HBsAg and HBeAg inserum might have X gene in serum and HBxAg expressionin carcinoma tissues. CONCLUSION:our date suggest that HBXAg and FaS/FasLSyst6m Plays an imPortant role in the develoPment of humanHCC.ExPression of HBXAg ean leads to exPression of Fas/fasL System whieh and reverse apoptosis of hepatoeellularcarcinoma induced by Fasl
AIM: To study the expression and serum level of HBxAg, Fas and FasL in tissues of HCC patients, and toassess therelationship between HBxAg and Fas / FasL system. METHODS: Tissues from 50 patients with HCC were tested for the expression of HBxAg, Fas and FasL by S-Pimmunohistochemistry. Levels of sFas / sFasL and HBsAg / HBeAg were measured by ELISA assay. HBV X gene was detected by PCR in serum and confirmed by automatic sequencing. Ftyty cases of liver cirrhosis and 30 normal controls were involved in serum analysis .RESULTS: The expression of HBxAg, Fas and FasL incarcinoma tissues was 96%, 84% and 98%, respectively. Of the patients with HBxAg, Fas and FasL was observed predominatelyin cytoplasms, no significant difference was found in intensity between HBxAg, Fas and FasL (P> 0.05) . HBxAg, Fas and FasL expressight in the same area of carcinoma tissues and thisco-expression could be found in most patients with HCC. These me levels of sFas in serum from HCC, cirrhosis and normal controls were 762.29 ± 391.56 μg · L -1 ), 835.36 ± 407.33 μg · L -1 and238.27 ± 135.29 μg · L -1 .The mean levels of sFasL in serum from HCC, cirrhosis and normal controls were 156.36 ± 9.61μg · L -1, , 173.63 ± 18.74 μg · L -1 and 121.96 ± 7.83 μg · L -1, respectively. Statistical analysis showed that both sFas and sFasL in HCC and cirrhosis patients were significantly higher than those in normal controls (P <0.01). Serum HBV X gene was found in 32% of HCC patients and 46% of cirrhotic patients. There was no significant relationship between serum level of sFas / sFasL and serum X gene detection (P> 0.05). Light of HCC patients with negative HBsAg and HBeAg inserum might have X gene in serum and HBxAg expressionin carcinoma tissues. CONCLUSION: our date suggest that HBXAg and FaS / FasLSyst6m Plays an imPortant role in the develoment of humanHCC. Exression of HBXAg ean leads to exPression of Fas / fasL System whieh and reverse apoptosis of hepatoeellularcarcinoma induced by Fasl