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目的:检测IL-1β与IL-10mRNA在正常牙龈及牙周炎患者牙龈组织中的表达,探讨二者与炎症的关系及内源性抗炎因子IL-10对致炎因子IL-1β是否有拮抗作用。方法:采集14例成人牙周炎患者炎症区牙龈组织,6例正常牙龈组织,利用逆转录-聚合酶链反应检测其中IL-1β与IL-10mRNA的表达及其强弱程度。结果:成人牙周炎组牙龈组织IL-1βmRNA的阳性表达率为92.86%,IL-10mRNA阳性表达率为71.43%,二者与正常对照组相比均有显著性差异;两者之间无明显相关性;两者与临床指标间亦无明显相关性。结论:致炎性和抗炎性细胞因子均可在牙龈组织中表达;机体自身IL-10水平不足以完全拮抗IL-1β活性;IL-10对致炎性细胞因子IL-1β的调控作用有待进一步研究。
OBJECTIVE: To detect the expression of IL-1β and IL-10 mRNA in gingival tissues of normal gingival and periodontitis patients and to explore their relationship with inflammation and whether IL-10, an endogenous anti-inflammatory cytokine IL- Antagonism. Methods: Gingival tissues from 14 patients with chronic periodontitis and normal gingival tissues from 6 patients were collected. The expression of IL-1β and IL-10 mRNA and their intensity were detected by reverse transcription-polymerase chain reaction. Results: The positive expression rate of IL-1β mRNA in gingival tissues of adult periodontitis group was 92.86%, the positive rate of IL-10 mRNA expression was 71.43%, both were significantly different from the normal control group Correlation between the two indicators and no significant correlation between clinical indicators. CONCLUSION: Both inflammatory and anti-inflammatory cytokines can be expressed in gingival tissues. The body’s own IL-10 level is not enough to completely antagonize the activity of IL-1β. The regulatory effect of IL-10 on IL-1β further research.