论文部分内容阅读
目的:建立金嗓清音丸中丹皮酚的含量测定方法。方法:采用高效液相色谱法测定该药中丹皮酚的含量。色谱柱为Agi-lent1100C18柱,流动相为甲醇-0.1%乙酸溶液(60∶40),流速为1.0mL·min-1,检测波长为274nm。结果:丹皮酚在0.688～22.000μg·mL-1(r=0.9998)浓度范围内与峰面积积分值线性关系良好;平均回收率为95.91%,RSD=2.81%。结论:该方法准确、可靠、专属性强,可用于金嗓清音丸的质量控制。
Objective: To establish a method for the determination of paeonol in Jintuizhengyin pill. Methods: The content of paeonol in this medicine was determined by high performance liquid chromatography. The column was Agi-lent1100C18 and the mobile phase was methanol-0.1% acetic acid solution (60:40) at a flow rate of 1.0 mL · min-1 with a detection wavelength of 274 nm. Results: Paeonol had a good linear relationship with the integral of peak area in the concentration range of 0.688 ~ 22.000μg · mL-1 (r = 0.9998). The average recovery was 95.91% and RSD was 2.81%. Conclusion: The method is accurate, reliable and specific, and it can be used for quality control of Jinshitong tone pills.