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目的对山东省原发性高血压(EH)患者群体的2号染色体进行扫描,寻找关联区域,定位易感基因。方法用DNA混合池(DNApooling)方法,在2号染色体上间隔10cM(厘摩)遗传距离选择30个微卫星遗传标记,对450例EH患者和450例正常对照者组成的DNA混合样本分别进行扫描。采用Clump软件进行统计学分析,比较患者组与对照组每个等位基因频率的差异。结果在D2S2211(2p25.1)位点患者组与对照组的等位基因频率差异有统计学意义(P<0.01)。结论山东省EH患者群体在2号染色体D2S2211位点存在关联,附近可能存在易感基因,需进一步筛查。
Objective To scan chromosome 2 of patients with essential hypertension (EH) in Shandong Province and find out the related regions and locate the susceptible genes. Methods A DNA pooling method was used to select 30 microsatellite markers from chromosome 2 on a genetic distance of 10 cM. DNA samples from 450 patients with EH and 450 healthy controls were screened separately. . Statistical analysis was performed using Clump software to compare the frequency of each allele between the patient group and the control group. Results There was a significant difference in allele frequencies between patients and controls at D2S2211 (2p25.1) (P <0.01). Conclusion The population of EH patients in Shandong Province is associated with the D2S2211 locus on chromosome 2, and there may be susceptible genes in the vicinity of the locus for further screening.