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罗伯茨绿僵菌Metarhizium robertsii是一种重要的广谱性杀虫真菌,在害虫的生物防治中应用广泛。研究表明microRNAs在丝状真菌的生长发育中可能发挥重要调控作用。迄今有关miRNAs在绿僵菌产孢过程中的作用还未见研究报道。本文分析了实验室前期鉴定的一个绿僵菌新miRNA(mro-miR-33)在绿僵菌产孢过程中的作用。结果表明,绿僵菌在不同培养条件下mro-miR-33的表达量有显著差异,且在孢子发育过程表达量下降。在野生菌株中过表达mro-miR-33后,绿僵菌的产孢量显著下降,qRT-PCR分析表明孢子发育关键调控基因brl A表达显著下调,而敲除mro-miR-33后,绿僵菌的产孢量显著增加。这些变化表明mro-miR-33在绿僵菌的产孢过程中发挥着重要的作用。
Metarhizium robertsii is an important broad-spectrum insecticidal fungus, widely used in the biological control of pests. Studies have shown that microRNAs may play an important regulatory role in the growth and development of filamentous fungi. So far, the role of miRNAs in sporulation of Metarhizium anisopliae has not been reported. In this paper, we analyzed the role of a new Metarhizium anisopliae miRNA (mro-miR-33) identified in the laboratory during sporulation of Metarhizium anisopliae. The results showed that the expression of mro-miR-33 in Metarhizium anisopliae was significantly different under different culture conditions, and the expression level of mro-miR-33 was decreased during spore development. After mro-miR-33 was overexpressed in the wild strain, the sporulation of Metarhizium anisopliae significantly decreased. QRT-PCR analysis showed that the expression of brl A, a key regulator of spore development, was significantly down-regulated. The amount of sporulation of Stibcuin increased significantly. These changes indicate that mro-miR-33 plays an important role in sporulation of Metarhizium anisopliae.