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在发现甘氨酸（ｇｌｙｃｉｎｅ，Ｇｌｙ）是有可能成为内毒素（ｅｎｄｏｔｏｘｉｎ，ＥＴ）拮抗剂的重要药物之一的基础上，从二个方面进一步观察了Ｇｌｙ拮抗ＥＴ的效应。（１）观察不同浓度的Ｇｌｙ对内毒素鲎试验凝胶形成的影响，发现随着Ｇｌｙ浓度的增高，拮抗效果越明显，００５％～０１％的Ｇｌｙ已显示出拮抗效应。半定量方法测出４ｇＧｌｙ大约拮抗０５μｇ内毒素；（２）观察Ｇｌｙ对ＥＴ诱导单核细胞产生ＥＰ的抑制效果。从兔血中提取单个核细胞，分别加入ＥＴ，ＥＴ＋Ｇｌｙ，Ｇｌｙ，在３７℃，体积分数为５％的ＣＯ２孵箱中培养。离心取上清，再分别经耳缘静脉注入６０只新西兰家兔体内，结果显示：甘氨酸明显拮抗内毒素诱导单核细胞产生ＥＰ。单纯ＥＴ培养的上清，ΔＴ为（０８４±０１４）℃，ＴＲＩ２为４２±０３４，而内毒素与甘氨酸作用后再与单个核细胞培养的上清组，ΔＴ为（０２７±００５）℃，ＴＲＩ２为１６４±０１４（Ｐ＜００１），单纯甘氨酸培养的上清液对体温无显著影响，平均体温反应曲线也反映出同样结果。
Based on the finding that glycine (Gly) is one of the important drugs that may become endotoxin (ET) antagonists, the effect of Gly antagonizing ET is further observed from two aspects. (1) The effects of different concentrations of Gly on the gel formation of the endotoxin test were observed. It was found that the more obvious the antagonistic effect was with the increase of Gly concentration, the 005% -01% Gly had antagonistic effect. Semiquantitative determination of 4gGly antagonistic about 0 5μg of endotoxin; (2) To observe the inhibitory effect of Gly on ET-induced monocyte EP. The mononuclear cells were extracted from rabbit blood and were respectively added ET, ET + Gly, Gly and cultured in a CO2 incubator with a volume fraction of 5% at 37 ° C. The supernatants were centrifuged and then injected into 60 New Zealand rabbits via the ear vein respectively. The results showed that glycine significantly antagonized endotoxin-induced monocyte EP. In the supernatant of ET alone, ΔT was (084 ± 014) ℃ and TRI2 was 42 ± 034, while ΔT was higher than that of mononuclear cells cultured with endotoxin and glycine (027 ± 005) ℃, TRI2 was 164 ± 014 (P <001), the pure glycine culture supernatant had no significant effect on body temperature, mean body temperature response curve also reflected the same result .