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在无细胞百日咳苗苗（APV）制备中，去除内毒素是非常必要的一步。目前国内外广泛应用的蔗糖密度梯度超离心法（SDGC）需持殊设备，操作繁琐．使其应用受到限制。为此．我们应用DE。。往层析取代SDGC，结果表明，用DE。。层析处理百日咳菌抗原提取物，其内毒素可减少99．9％，
In the preparation of acellular pertussis vaccine (APV), the removal of endotoxin is a very necessary step. Currently widely used sucrose density gradient ultracentrifugation (SDGC) at home and abroad need special equipment, cumbersome operation. Its application is limited. to this end. We apply DE. . To replace the SDGC chromatography, the results show that using DE. . Chromosome treatment of pertussis antigen extract, the endotoxin can be reduced by 99.9%