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目的利用不同方法建立兔VX2移植肝癌模型,选择最佳的建模方法。方法45只新西兰大白兔随机被分成3组,每组15只,分别采用开腹肝内VX2瘤块包埋法(A组)、CT定位下经皮穿刺肝脏后注射VX2瘤块悬液法(B组)、CT定位下经皮穿刺肝脏后推送VX2瘤块法(C组)制作肝癌模型,比较各组动物种植成功率及存活时间。结果A、B、C各组动物种植后3周的死亡率分别为26.7%、20.0%、0.0%,A、B两组与C组的差异有统计学意义(P<0.05)。A、B、C各组动物种植后3周的成瘤率分别为53.3%、80.0%、93.3%,A组与B、C两组的差异有统计学意义(P<0.05)。A、B、C组动物的平均生存期分别为(25.5±19.4)d(、37.5±15.7)d(、48.7±11.1)d,差异有统计学意义(P<0.05)。所有种植成功的模型都得到病理学证实。结论成功建立了兔VX2肝癌模型;CT定位下经皮穿刺直接推送瘤块法的建模成功率明显高于其他两种方法。
Objective To establish a rabbit model of VX2 liver cancer by different methods and choose the best modeling method. Methods Forty five New Zealand white rabbits were randomly divided into three groups (n = 15 each). The rabbits were randomly divided into 5 groups: open VX2 tumor embedding (group A), percutaneous hepatic embolization Group B). After liver was punctured with CT, the model of liver cancer was established by pushing VX2 tumor mass (Group C). The success rate and survival time of each group were compared. Results The mortality rates of three weeks after planting in groups A, B and C were 26.7%, 20.0% and 0.0%, respectively. The differences between groups A and B were statistically significant (P <0.05). The tumorigenic rates of A, B and C groups were 53.3%, 80.0% and 93.3% after 3 weeks of implantation, respectively. There was significant difference between group A and group B and group C (P <0.05). The mean survival time of group A, B and C was (25.5 ± 19.4) d (37.5 ± 15.7) d (48.7 ± 11.1) d, respectively, with statistical significance (P <0.05). All successful planting models have been pathologically confirmed. Conclusion The rabbit model of VX2 liver cancer has been successfully established. The success rate of modeling tumor directly by percutaneous puncture under CT localization is obviously higher than that of the other two methods.