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目的探讨维康颗粒(Weikang Granule,WG)对乳腺癌小鼠肿瘤相关性疲劳的影响及代谢机制。方法将40只成功接种4T1乳腺肿瘤细胞的小鼠分为肿瘤对照TC组(生理盐水灌胃,20 m L/kg,1次/日)、维康颗粒(WG)组、紫杉醇(PTX)组、维康颗粒+紫杉醇(WG+PTX)组,每组10只,给药组分别给予相应WG(6g/kg,灌胃,1次/日)及PTX(10 mg/kg,腹腔注射,隔日1次),连续干预21日。观察干预前及干预第10、20日力竭游泳时间。干预结束后取血,应用核磁共振氢谱(1H-NMR)技术获取小鼠代谢指纹图谱信息,采用PCA、PLSDA、OPLS-DA进行数据处理,观察各组血浆差异代谢物。结果与TC组比较,PTX组力竭游泳时间减少(P<0.01),瓜氨酸水平升高,肌醇、磷酸胆碱、异柠檬酸、甘油磷酸酯、亚油酸、谷氨酰胺、辅酶A、天冬氨酸、丝氨酸、高半胱氨酸、赤藓糖水平下降(P<0.01,P<0.05);WG组力竭游泳时间延长(P<0.01,P<0.05),甘油磷酸酯水平升高(P<0.01)。与PTX组比较,WG+PTX组力竭游泳时间延长(P<0.01),神经酸、辅酶A、磷酸烯醇丙酮酸、肌醇、赤藓糖水平升高(P<0.01,P<0.05)。结论维康颗粒具有减轻化疗相关性疲劳的作用,其机制与促进ATP生成,并加强磷酸戊糖途径、脂肪酸代谢、糖异生相关。
Objective To investigate the effect of weikang granule (WG) on tumor-related fatigue in mice with breast cancer and its metabolic mechanism. Methods Forty mice successfully inoculated with 4T1 mammary tumor cells were divided into two groups: tumor control TC group (20 mL / kg once daily), WG group, PTX group (10 mg / kg, intraperitoneal injection, every other day), Wick granules + paclitaxel (WG + PTX) 1), continuous intervention on the 21st. Observed before intervention and intervention on the 10th, 20th exhausted swimming time. Blood samples were obtained after the intervention. Metabolic fingerprint of mice was obtained by 1H-NMR. PCA, PLSDA and OPLS-DA were used for data processing, and plasma differential metabolites of each group were observed. Results Compared with TC group, the swimming time of exhausted group in PTX group was decreased (P <0.01), and the level of citrulline increased. Inositol, phosphocholine, isocitrate, glycerophosphate, linoleic acid, glutamine, A, aspartic acid, serine, homocysteine and erythrose decreased significantly (P <0.01, P <0.05) Elevated levels (P <0.01). Compared with the PTX group, the prolonged swimming time in WG + PTX group was prolonged (P <0.01), the level of neuron acid, coenzyme A, phosphoenolpyruvate, inositol and erythrose increased (P <0.01, P < . Conclusion Weikang Granule has the effect of relieving chemotherapy-related fatigue. Its mechanism is related to the promotion of ATP production, enhancement of pentose phosphate pathway, fatty acid metabolism and gluconeogenesis.