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目的:研究东亚钳蝎毒素Martentoxin对脐静脉内皮细胞释放NO的影响。方法:人脐带经胶原酶消化分离后获得脐静脉内皮细胞,经抗血管性血友病8因子鉴定后,用TNF-α建立NO释放模型,观察Martentoxin对脐静脉内皮细胞释放NO的影响。结果:①经6,12,24h作用后,10μM、100μM Martentoxin能降低TNF-α引起的NO释放增加(P<0.05)。②Martentoxin降低TNF-α引起的iNOS活性增强并对TNF-α引起的内皮源性一氧化氮合酶mRNA下调具有一定保护作用(P<0.05)。结论:Martentoxin抑制TNF-α引起的脐静脉内皮细胞释放NO增加。
Objective: To investigate the effect of Martentoxin, a toxin from East Asian bee venom, on the release of nitric oxide from human umbilical vein endothelial cells. Methods: Umbilical vein endothelial cells were obtained after human umbilical cord collagenase digestion and separation. After identification by anti-von Willebrand factor, the NO release model was established by TNF-α, and the effect of Martentoxin on NO release from umbilical vein endothelial cells was observed. Results: ① After 6, 12 and 24 hours, 10μM and 100μM Martentoxin decreased the release of TNF-α-induced NO (P <0.05). ②artentoxin reduced the iNOS activity induced by TNF-α and enhanced the expression of endogenous nitric oxide synthase mRNA induced by TNF-α (P <0.05). Conclusion: Martentoxin inhibits TNF-α-induced NO release from human umbilical vein endothelial cells.