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目的通过高效氯氰菊酯对大鼠血液中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量影响的检测,探讨其可能的毒作用机制。方法将雄性Wistar大鼠随机分为4组,即高效氯氰菊酯高、中、低剂量组和对照组。经口灌胃30 d,观察大鼠一般状况及体重的变化,实验结束后腹主动脉采血检测血清中SOD、GSH-Px活力和MDA含量;取脑、肝、肾、脾脏、睾丸组织称重,计算脏器系数,部分组织放于10%甲醛中固定待做病理组织学检查。结果高效氯氰菊酯可引起大鼠体重降低,实验第4周体重高剂量组(281.43±15.87)g,中剂量组(295.55±14.60)g,低剂量组(304.81±14.78)g,对照组(315.29±17.62)g,染毒组分别与对照组比较,差异均有统计学意义(P<0.05);脑/体比和睾/体比增加,高剂量组为(0.47±0.03)、(1.17±0.14),中剂量组为(0.46±0.03)、(1.20±0.11),低剂量组为(0.45±0.02)、(1.12±0.07),对照组为(0.43±0.03)、(1.08±0.11),染毒组分别与对照组比较,差异均有统计学意义(P<0.05);随着染毒剂量的增加,大鼠血清中SOD、GSH-Px活力逐渐降低,高剂量组为(34.57±1.83)U/ml、(880.83±55.73)μmol/L,中剂量组为(43.69±1.83)U/ml、(1 014.18±41.69)μmol/L,低剂量组为(54.42±1.82)U/ml、(1 129.72±31.17)μmol/L,对照组为(65.46±1.53)U/ml、(1 177.62±54.89)μmol/L;而MDA含量逐渐升高,高剂量组为(6.35±0.45)nmol/ml,中剂量组为(4.92±0.35)nmol/ml,低剂量组为(3.67±0.43)nmol/ml,对照组为(2.40±0.57)nmol/ml,染毒组分别与对照组比较,差异均有统计学意义(均P<0.05)。结论氧化性损伤可能是高效氯氰菊酯毒作用机制之一。
Objective cypermethrin on rats blood super oxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and malondialdehyde Effect (MDA) content detection investigate the possible mechanism of toxicity . Methods Male Wistar rats were randomly divided into 4 groups, namely, beta-cypermethrin high, middle and low dose groups and control group. After oral administration for 30 days, the general condition and body weight of the rats were observed. After the experiment, serum of the abdominal aorta was collected to measure the activity of SOD, GSH-Px and the content of MDA. The brain, liver, kidney, spleen and testis were weighed , Calculate the organ coefficient, part of the tissue placed in 10% formaldehyde fixed to be done histopathological examination. Results cypermethrin can cause a decrease in body weight of rats, four weeks of the experiment body weight of the high dose group (281.43 ± 15.87) g, middle dose group (295.55 ± 14.60) g, low dose group (304.81 ± 14.78) g, in the control group (315.29 ± 17.62) g, respectively. Compared with the control group, the difference was statistically significant (P <0.05). The ratio of brain / body and the ratio of testis to body increased in high dose group (0.47 ± 0.03) (0.46 ± 0.03) and (1.20 ± 0.11) in the middle dose group and (0.45 ± 0.02) and (1.12 ± 0.07) in the low dose group and (0.43 ± 0.03) and (1.08 ± 0.11) in the control group Compared with the control group, the differences were statistically significant (P <0.05). With the increase of exposure dose, the activities of SOD and GSH-Px in the serum of rats in the high dose group were (34.57 ± 1.83) U / ml and (880.83 ± 55.73) μmol / L respectively, and the middle dose group was (43.69 ± 1.83) U / ml and (014.18 ± 41.69) μmol / L and the low dose group was 54.42 ± 1.82 U / 1 129.72 ± 31.17) μmol / L, the control group (65.46 ± 1.53) U / ml, (1 177.62 ± 54.89) μmol / L; and MDA content increased, the high dose group (6.35 ± 0.45) nmol / ml (4.92 ± 0.35) nmol / ml in the middle dose group, (3.67 ± 0.43) nmol / ml in the low dose group, and (2.40 ± 0.57) nmol / ml in the control group The differences between the two groups were statistically significant (all P <0.05). Conclusion Oxidative damage may be one of the mechanisms of alpha-cypermethrin toxicity.