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目的:优化槐耳/板蓝根双向发酵条件并确定发酵终点。方法:以菌质多糖含量为响应值,采用响应面法考察温度、相对湿度、接种量对槐耳/板蓝根双向发酵条件的影响,同时确定槐耳/板蓝根菌质双向发酵终点。利用紫外分光光度法测定菌质多糖含量。结果:槐耳板蓝根菌质多糖的最佳发酵条件为温度29.4℃,接种量0.826 mL·g-1,相对湿度75.5%,菌质多糖质量分数11.496%,与预测值(11.548%)偏差0.45%,二项式拟合复相关系数0.9826,所得回归模型拟合情况良好,达到设计要求;确定发酵终点20 d。结论:响应面法可用于优选槐耳/板蓝根双向发酵条件,具有简便、预测性良好等优点。
Objective: To optimize the two-way fermentation conditions and determine the end of fermentation. Methods: The effect of temperature, relative humidity and inoculum on the bi-directional fermentation of Sophora flavescens / Radix isatidis was investigated by response surface methodology (RSM), and the biotrophic end point was determined. Determination of Bacterial Polysaccharide by UV Spectrophotometry. Results: The optimum fermentation conditions were as follows: temperature 29.4 ℃, inoculum 0.826 mL · g-1, relative humidity 75.5%, polysaccharides mass fraction 11.496%, which was 0.45% different from the predicted value (11.548%), , The binomial fitted complex correlation coefficient 0.9826, the regression model fitting well, to meet the design requirements; determine the fermentation end point 20 d. Conclusion: Response surface methodology can be used to optimize bi-directional fermentation conditions of Sophora flavescens / Radix isatidis, which is simple and predictive.