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大鼠颈动脉注射１％荧光素钠（ＦｌＮａ），按不同时间点取心、脑、肝、肾等九个脏器，利用荧光分光光度计测定各脏器组织匀浆液的荧光强度，得到不同脏器中ＦｌＮａ的分布及ＦｌＮａ在不同脏器中随时间的衰减变化趋势，并辅以冰冻切片进行观察。实验发现，不同脏器中ＦｌＮａ的分布极不均匀，以脑为最低，肝、肾为最高，冰冻切片也显示了同样的分布差别。这些结果表明我们所建立的方法可以定量地反映ＦｌＮａ在不同脏器微血管的通透情况
Rat carotid artery injection of 1% sodium fluorescein (FlNa), at different time points coring, brain, liver, kidney and other nine organs, the use of fluorescence spectrophotometer determination of the fluorescence intensity of the homogenates of various organs, get different The distribution of FlNa in organs and the tendency of FlNa decaying with time in different organs were observed and supplemented with frozen sections. The experiment found that the distribution of FlNa in different organs is very uneven, the brain is the lowest, liver and kidney are the highest, frozen sections also show the same distribution differences. These results indicate that the method we established can quantitatively reflect the FlNa permeability of microvessels in different organs