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目的:Ghrelin是一种主要由胃粘膜产生的28氨基酸肽。因为血浆中的ghrelin完全依赖于摄食获得,所以这种激素对食欲和能量平衡有显著影响。本研究侧脑室注射ghrelin后两小时,通过检测食物容器内剩余食物及水的量,观察中枢应用ghrelin对进食的影响。方法:每天侧脑室注射一次ghrelin(0.4μg),连续注射4天。每天测量体重,同时实验结束后检测腹膜后、附睾脂肪(WAT)含量以及血液中瘦素和胰岛素水平。结果:在第二次侧脑室注射ghrelin后,大鼠第一个和第二个30分钟时间段内进食与对照组相比显著增多(P<0.05),分别为47.2%和63.8%。自第二次注射ghrelin直至治疗结束,大鼠体重显著增加(P<0.05),食物和水的摄入量显著增多(P<0.05)。实验结束后,大鼠腹膜后和附睾WAT含量显著增加(231.4%,P<0.01;84.1%,P<0.05);血清胰岛素水平显著升高(42.3%,P<0.05),而血清瘦素水平显著下降(74.1%,P<0.05)。结论:中枢多次注射ghrelin对自由摄食的成年大鼠摄食具有促进作用。
Purpose: Ghrelin is a 28 amino acid peptide mainly produced by the gastric mucosa. Because ghrelin in plasma is completely dependent on ingestion, this hormone has a significant effect on appetite and energy balance. In this study, two hours after ghrelin injection in the lateral ventricle, the amount of food and water remaining in the food container was measured to observe the effect of central ghrelin on food intake. Methods: Intracerebroventricular injection of ghrelin (0.4μg) once daily for 4 days. Body weight was measured daily, and the peritoneum, epididymal fat (WAT) content, and leptin and insulin levels in the blood were measured after the experiment. RESULTS: After the second intracerebroventricular injection of ghrelin, rats in the first and second 30-minute time periods had significantly increased (P <0.05) 47.2% and 63.8%, respectively. From the second injection of ghrelin until the end of treatment, the body weight of rats increased significantly (P <0.05), and food and water intake increased significantly (P <0.05). At the end of the experiment, the WAT content of retroperitoneal and epididymis was significantly increased in rats (231.4%, P <0.01; 84.1%, P <0.05); serum insulin level increased significantly (42.3%, P <0.05) Significantly decreased (74.1%, P <0.05). Conclusion: Multiple central ghrelin injections can promote the feeding of rats fed freely.