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Objective:To investigatetherolesof thepolarizedpopulationsof T helpercellsisolatedfromtheperipheral bloodmononuclearcells(PBMCs)of individualswithchronichepatitisB virus(HBV)infection.Methods:PBMCsfrom patientswithchronicHBVinfectionwereseparatedroutinely,stimulatedby PMA,ionomycinandmonensin,andthepro-ductionof IL-4,IFN-γandTGF-βby CD4 + T cellswas observedby flowcytometry(FACS).Results:Thepercentagesof theT cellsproducingIFN-γ,IL-4or TGF-βrangedfrom2.3%to18.6%,1.1%to8.7%and0.7%to7.1%respectivelya-mongCD4 + cellsfromnon-infectedindividuals.Themajorityof CD4 + T cellsin PBMCsfromindividualswithchronic HBVinfectionwereTh0cells.Theproportionof Th1cellsinpatientswithactivechronichepatitisB washigherthanthat in patientsat inactivestageof thedisease(P<0.05),indicatinga significantelevationof Th1cellswiththehepaticinflam-mationactivity.Thepercentageof Th2cellsinindividualswithHBVinfectionwashigherthanthatincontrols(P<0.05),butshowedno differencebetweendifferentpatients(P>0.05).Thepercentageof Th3cellswas higherin asymptomatic HBVcarriersthanthatinpatientswithchronichepatitisB andinhealthycontrols(P<0.05).Conclusions:Th1-typecy-tokinesarerelatedwithhepaticinflammationactivityof chronichepatitisB,andTh2cellsmaybe associatedwiththeper-sistenceof HBVinfection.Th3cellscooperatingwithTh2cellsarelikelyto functionas negativeimmunoregulator,and maybe responsiblefortheimmunetolerancestateof chronicHBVinfection.
Objective: To investigatetherolesof thepolapoptoppopulations of T helper cells are derived from theperipheral blood mononuclear cells (PBMCs) of individuals with chronic hepatitis B virus (HBV) infection. Methods: PBMCs from patients with chronicHBV infection complete and classified cytokinely, stimulatedby PMA, ionomycinandmonensin, andthepro- ductionof IL- 4, IFN- γ and TGF- βby CD4 + T cellswas observedby flowcytometry (FACS) . Results: The per centages of the T cellsproducing IFN-γ, IL-4or TGF-βrangedfrom2.3% to18.6%, 1.1% to 8.7% and0.7% to7.1% respectivelya-mongCD4 + cellsfromnon-infectedindividuals.Themajorityof CD4 + T cellsin PBMCs from in patients with chronic HBV infection in T h0 cells. The proportion of Th1 cells in patients with chronic choroidal hepatitis B washigherthanthat in patients with inactive stage of the disease (P <0.05), indicating a significant elevation of Th1 cells with the presence of Thrombocytosis in malarial patients ts (P> 0.05) .Thepercentageof Th3cellswas higherin asymptomatic HBVcarriersthanthatinpatientswithchronichepatitisB andinhealthycontrols (P <0.05) .Conclusions: Th1-typecy-tokinesarerelatedwithhepaticinflammationactivityof chronichepatitisB, andTh2cellsmaybe associatedwiththeper-sistenceof HBVinfection.Th3cellscooperatingwithTh2cellsarelikelyto functionas negativeimmunoregulator, and maybe responsiblefortheimmunetolerancestateof chronicHBVinfection.