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目的:研究脂肪源干细胞能否与牙周膜成纤维细胞直接共培养,在共培养状态下,脂肪源干细胞能否促进牙周膜成纤维细胞胶原特异基因的表达。方法:分别从新西兰白兔皮下脂肪和牙周膜组织中分离出脂肪源干细胞和牙周膜成纤维细胞,体外常规培养;将两种细胞等量混合共培养,利用光镜和F-actin染色标记细胞骨架的方法观察共培养细胞的形态变化,并与单纯培养的细胞比较;利用DAPI染色标示细胞核细胞计数法检测共培养细胞的增殖情况;利用定量PCR法检测特异基因I型胶原在单纯培养和共培养细胞中的表达。结果:脂肪源干细胞和牙周膜成纤维细胞能直接共培养;共培养细胞形态近似牙周膜成纤维细胞;共培养对细胞增殖无明显影响;脂肪源干细胞可提高牙周膜成纤维细胞I型胶原基因的表达。结论:脂肪源干细胞可与牙周膜成纤维细胞共培养,并促进牙周膜成纤维细胞的胶原分泌。
OBJECTIVE: To investigate whether adipose-derived stem cells can directly co-culture with periodontal ligament fibroblasts and whether adipose-derived stem cells can promote the expression of collagen-specific genes in periodontal ligament fibroblasts. Methods: Adipose derived stem cells and periodontal ligament fibroblasts were isolated from subcutaneous adipose tissue and periodontal ligament tissue of New Zealand white rabbits respectively and cultured in vitro. The two kinds of cells were co-cultured in the same amount and stained with light microscope and F-actin The morphological changes of cocultured cells were observed by cytoskeleton labeling and compared with that of cultured cells. The proliferation of cocultured cells was detected by DAPI staining. The specific gene type I collagen was detected by quantitative polymerase chain reaction And co-cultured cells. RESULTS: ADSCs could differentiate into periodontal ligament fibroblasts directly. Cocultured cells were similar to periodontal ligament fibroblasts. Co-culture had no significant effect on cell proliferation. Adipose-derived stem cells could enhance periodontal ligament fibroblast I Type collagen gene expression. Conclusion: Adipose-derived stem cells can co-culture with periodontal ligament fibroblasts and promote collagen secretion of periodontal ligament fibroblasts.