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A novel method is developed for the determination of cefradine by using sodium nitroprusside as chromogenic reagent.The experiment indicates that a russety product is formed by the reaction of cefradine with sodium nitroprusside in basic solution,and the maximum absorption wavelength (λ_(max)) of russety product is 505 nm.And the sensitization of tetradeeyl benzyl dimethyl ammonium chloride for the reaction of cefradine with sodium nitroprusside is remarkable.The apparent molar absorption coefficient (ε_(505)) is 2.81×10~3 L/mol cm.The linear equation is A = 0.0657 + 0.00804C (μg/mL) in the range of 1.50--55.0 wg/mL of cefradine with a correlation coefficient r=0.9992,and the detection limit is 1.38μg/mL.This method has been applied to determine cefradine in capsule and tablet samples.
A novel method is developed for the determination of cefradine by using sodium nitroprusside as chromogenic reagent. The experiment indicates that a russety product is formed by the reaction of cefradine with sodium nitroprusside in basic solution, and the maximum absorption wavelength (λ_ (max)) of russety product is 505 nm. And the sensitization of tetradeeyl benzyl dimethyl ammonium chloride for the reaction of cefradine with sodium nitroprusside is remarkable. The apparent molar absorption coefficient (ε_ (505)) is 2.81 × 10 -3 L / mol cm. linear equation is A = 0.0657 + 0.00804C (μg / mL) in the range of 1.50 - 55.0 wg / mL of cefradine with a correlation coefficient r = 0.9992 and the detection limit is 1.38 μg / mL. This method has been applied to determine cefradine in capsule and tablet samples.