论文部分内容阅读
本文用光学镜细胞化学方法与电子显微镜技术,用100R或300R~(60)Co γ射线对大白鼠进行一次全身照射,照射后以不同时间对淋巴细胞核形态及LDH反应的观察。其结果,细胞核破损率和LDH从胞核中以不同形式泄逸的反应率,在时相曲线上表现出平行关系的规律性。细胞化学彩色照片直观地揭示了LDH在淋巴细胞核中的存在。酶反应在胞核内的行为和向外泄逸的现象反映了淋巴细胞核受电离辐射后,内含酶发生动态反应。而细胞核异型化的亚显微结构变化,验证了动态发展过程。我们以动力学概念对辐射致胞核损伤与死亡的原发过程提出解说。
In this paper, the optical microscope cytochemistry and electron microscopy techniques, using 100R or 300R ~ (60) Co γ-rays of a rat whole body irradiation, irradiation at different times on lymphocyte nuclear morphology and LDH response observed. As a result, the rate of nuclear damage and the rate of LDH leakage from the nucleus in different forms showed a regular pattern of parallel relationships in the phase-to-phase curve. Cytochemical color photographs intuitively reveal the presence of LDH in the lymphocyte nuclei. Enzyme reactions in the nucleus of the behavior and the phenomenon of outward leakage reflects the lymphocyte nuclei by ionizing radiation, the enzyme containing dynamic reaction occurs. The sub-microstructural changes of the nucleus heterozygosity verified the dynamic development process. We use kinetic concepts to explain the primary process of radiation-induced nuclear damage and death.