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Pyrus pashia Buch-Ham, a wild specie was used to investigate the physiological effects of irondeficiency in culture solution. The result showed that Chla, Chlb, total chlorophyll content and photosynthesisrate(Ph) decreased sharply, and the decrease of Pn was prior to that of Chl content under the iron deficiency.The iron deficiency symptoms were visible when the iron concentration in culture medium was less than 25μmol L-1. Peroxidase(POD) and catalase(CAT) activity in iron-deficient leaves declined significantly, andPOD was more sensitive than CAT to Fe deficiency. However, the positive correlation between CAT activityand Chl content was more significant than that between POD activity and Chi content. The content of nutrientelements in Fe-deficient leaves, which changed irregularly, were higher than that in normal leaves. There werea most significant positive correlation between active Fe and Chi content, and between active Fe and Pn respec-tively. Therefore, active Fe could be useful physiological predicting index for diagnosis.