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作者用不同密度的彭亨丝虫微丝蚴人工感染5个不同地理株的致乏库蚊。致乏库蚊除马来亚株外,还有来源于利物浦的4个选育品系,即坦桑尼亚株、上沃尔特株、尼日利亚株及委内瑞拉株。各株蚊虫经饲养2～3代后的成蚊,穿过3～10天小鸡皮肤作成的薄膜刺吸含彭亨丝虫微丝蚴的阳性血液(血液内加肝素),供血动物体内微丝蚴的密度幅度为每立方毫米1.3～20.4条。将饱血蚊虫饲养10～12天后进行解剖,并分别记录蚊虫的头、胸和腹各部位的幼丝虫数目和发育期别。
The authors used 5 different geographic strains of Culex pipiens pallens to infect microflora of Paenia alba with different densities. Culex pipiens pallens, except for Malaya strains, there are four breeding strains derived from Liverpool, that is, Tanzania strain, Walter strain, Nigeria strain and Venezuela strain. Mosquitoes of each strain after feeding 2 to 3 generations of adult mosquitoes, 3 to 10 days through the skin of chickens made thin film containing penicillium filariasis microfilaria of the positive blood (blood plus heparin), blood-feeding animals in vivo microfilariae The density of the density range of 1.3 to 20.4 per cubic millimeter. The hematopoietic mosquitoes were kept for 10 to 12 days and then dissected. The numbers and developmental stages of young worm in the head, chest and abdomen were recorded.