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目的:检测宫颈上皮内瘤变及宫颈癌组织中CK17的表达,并探讨其临床意义。方法:应用免疫组织化学SP法,检测15例正常宫颈组织、15例宫颈上皮内瘤变(CIN)Ⅰ、17例CINⅡ、21例CINⅢ、36例宫颈鳞癌和8例宫颈腺癌组织中CK17的表达情况。结果:CK17在正常宫颈组织、CIN和宫颈鳞癌中的阳性表达率分别为0、56.6%和83.3%,3组相比差异有统计学意义,χ2=30.177,P<0.05。CINⅠ、CINⅡ和CINⅢ中,CK17的阳性表达率分别为20.0%、64.7%和76.2%,CINⅡ组阳性率高于CINⅠ组,差异有统计学意义,P=0.016;CINⅢ组阳性率高于CINⅠ组,差异有统计学意义,P=0.002;CINⅡ组和CINⅢ组阳性率差异无统计学意义,P>0.05。随着宫颈病变程度的加重,CK17的阳性表达率逐渐增高,其阳性表达同宫颈病变程度呈正相关,r=0.958,P<0.05。结论:CK17可作为高级别CIN和浸润性鳞状细胞癌诊断及预测病变进展趋势的标志。
Objective: To detect the expression of CK17 in cervical intraepithelial neoplasia and cervical cancer and to explore its clinical significance. Methods: Immunohistochemical SP method was used to detect the expression of CK17 in 15 cases of normal cervical tissue, 15 cases of cervical intraepithelial neoplasia (CIN) Ⅰ, 17 cases of CINⅡ, 21 cases of CIN Ⅲ, 36 cases of cervical squamous cell carcinoma and 8 cases of cervical adenocarcinoma. The expression of the situation. Results: The positive rates of CK17 in normal cervical tissue, CIN and cervical squamous cell carcinoma were 0, 56.6% and 83.3%, respectively. There was significant difference between the three groups (χ2 = 30.177, P <0.05). The positive rates of CK17 in CINⅠ, CINⅡand CINⅢwere 20.0%, 64.7% and 76.2% respectively. The positive rate in CINⅡgroup was higher than that in CINⅠgroup (P = 0.016), the positive rate in CINⅢgroup was higher than that in CINⅠgroup , The difference was statistically significant, P = 0.002; There was no significant difference in the positive rates between CINⅡgroup and CINⅢgroup (P> 0.05). With the severity of cervical lesions, the positive expression rate of CK17 gradually increased, and its positive expression was positively correlated with the degree of cervical lesions (r = 0.958, P <0.05). Conclusion: CK17 can be used as a marker for the diagnosis and prognosis of high grade CIN and invasive squamous cell carcinoma.