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角膜刮片细胞学检查常用Giemsa染色。但在真菌性角膜溃疡刮片检查中当涂片过厚或菌丝衰老时常不易分辨菌丝形态或遗漏诊断。Grocott六胺银染色真菌细胞壁及中隔清晰着染对比明显是病理学检查真菌敏感性比较高方法之一,但染色时间较长,操作较复杂,且不能观察组织细胞等细微形态变化。我们使用临床怀疑真菌性角膜溃疡
Corneal smear cytology commonly used Giemsa staining. But in fungal corneal ulcer smear smears too thick or mycelial senescence often difficult to distinguish mycelium morphology or omission diagnosis. Grocott hexamine silver stained fungus cell wall and septum clearly contrasting contrast is one of the pathological fungi detection method is relatively high, but the staining time is longer, the operation is more complicated, and can not observe the microstructure and other subtle morphological changes. We use clinically suspected fungal corneal ulcers