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目的:探讨玻璃化冷冻方法在冷冻保存卵母细胞中的临床应用。方法:选择2007年10月～2009年1月在我中心行卵母细胞玻璃化冷冻,并于2008年1月～2009年4月行卵母细胞玻璃化解冻的21例移植周期,对其复苏率、受精率及临床妊娠率进行统计。结果:共冷冻203枚卵母细胞,解冻后存活137枚(67.5%,137/203),对其中98枚成熟卵母细胞行单精子卵浆内显微注射,76枚(77.6%,76/98)正常受精,8例(38.1%,8/21)获得临床妊娠,其中6例单胎妊娠,2例3胎妊娠,并进行了减胎术。目前,1例已成功分娩,1例早期流产,6例正在妊娠中。结论:玻璃化冻融卵母细胞是一种方便可行的方法,可以获得较高的临床妊娠率。
Objective: To investigate the clinical application of vitrification method in cryopreservation of oocytes. Methods: From October 2007 to January 2009, we performed vitrification of oocytes in our center and performed 21 cases of oocyte vitrification thawing from January 2008 to April 2009, Rate, fertilization rate and clinical pregnancy rate statistics. Results: A total of 203 oocytes were frozen and 137 (67.5%, 137/203) survived thawed. Among 98 mature oocytes, 98) were normal fertilized, and 8 cases (38.1%, 8/21) received clinical pregnancy, of which 6 were singletons and 2 were third pregnancies. At present, one case has been successfully delivered, one case of early abortion, and six cases of pregnancy. Conclusion: Vitrification and oozing of oocytes is a convenient and feasible method for obtaining high clinical pregnancy rate.