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我们承担了“大鼠杂交瘤技术的建立及应用的研究”,已初步获得了成功。大鼠杂交瘤体系具有诸多优点,为小鼠杂交瘤体系的良好补充。其快速特异,简便易行的单克隆抗体(McAb)提纯方法,是明显优于小鼠的特点之一。纯化原理为Lou大鼠免疫球蛋白(Ig)轻链95％为Kappa型,其K轻链恒定区有二个不同个体基因型(1a、1b),定位稳定,二种鼠完全组织相容。利用小鼠抗Lou大鼠K-1a型轻链McAb
We assume the “rat hybridoma technology and the application of the establishment of research,” has initially been a success. The rat hybridoma system has many advantages and is a good complement to the mouse hybridoma system. Its rapid and specific, simple and easy method of purification of monoclonal antibodies (McAb) is clearly one of the characteristics of mice. Purification principle: Lou rat immunoglobulin (Ig) light chain 95% of Kappa type, K light chain constant region has two different genotypes (1a, 1b), positioning stable, two kinds of mice completely tissue compatible. Mouse anti-Lou rat K-la type light chain McAb was used